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rdh10 enu point mutation mice  (Genentech inc)

 
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    Genentech inc rdh10 enu point mutation mice
    Retinoic acid regulates β-catenin expression in brain endothelial cells. (A) Immunofluorescent images of coronal forebrain sections from E13.5 Wild-type ( <t>Rdh10</t> +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ) mutants stained for β-catenin (green), Isolectin-b4 (Ib4; red), and DAPI (blue). Scale bars are 200 μm. (B,C) Immunohistochemical images of (B) thalamic (thal) regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ; inset from A ) and Rdh10 ( Rdh10 -/- ; inset from A ) mutants and (C) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl brains stained for β-catenin (green) and Ib4 (red). Arrows indicate positive β-catenin expression within the Ib4 + vasculature. Scale bars are 50 μm. (B’,C’) Quantification and analyses (Student’s t -test) for percent of β-catenin expression within the total Ib4 + labeled blood vessels from (B’) Wild-type (gray; n = 6 animals) and Rdh10 mutant (black; n = 5 animals) and (C’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 5 animals). (D) Immunocytochemistry of β-catenin (green) expression in bEnd.3 cells following 48 h of vehicle, 50 nM RA, 1 μM RARi (AGN-194310), and RA + RARi. Scale bars are 50 μm. (D’) Quantification and analyses (ANOVA with Tukey’s post hoc analysis) on fluorescent intensity of β-catenin expression normalized to total number of DAPI+ bEnd.3 cells per field in vehicle (white), RA (black), RARi (gray), RA+RARi (black and gray) treated cells ( n = 3 independent experiments).
    Rdh10 Enu Point Mutation Mice, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rdh10+enu+point+mutation+mice/rdh10+enu+point+mutation+mutant+allele/pmc06290079-23-1-12
    Average 90 stars, based on 1 article reviews
    rdh10 enu point mutation mice - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Retinoic Acid Regulates Endothelial β-catenin Expression and Pericyte Numbers in the Developing Brain Vasculature"

    Article Title: Retinoic Acid Regulates Endothelial β-catenin Expression and Pericyte Numbers in the Developing Brain Vasculature

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2018.00476

    Retinoic acid regulates β-catenin expression in brain endothelial cells. (A) Immunofluorescent images of coronal forebrain sections from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ) mutants stained for β-catenin (green), Isolectin-b4 (Ib4; red), and DAPI (blue). Scale bars are 200 μm. (B,C) Immunohistochemical images of (B) thalamic (thal) regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ; inset from A ) and Rdh10 ( Rdh10 -/- ; inset from A ) mutants and (C) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl brains stained for β-catenin (green) and Ib4 (red). Arrows indicate positive β-catenin expression within the Ib4 + vasculature. Scale bars are 50 μm. (B’,C’) Quantification and analyses (Student’s t -test) for percent of β-catenin expression within the total Ib4 + labeled blood vessels from (B’) Wild-type (gray; n = 6 animals) and Rdh10 mutant (black; n = 5 animals) and (C’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 5 animals). (D) Immunocytochemistry of β-catenin (green) expression in bEnd.3 cells following 48 h of vehicle, 50 nM RA, 1 μM RARi (AGN-194310), and RA + RARi. Scale bars are 50 μm. (D’) Quantification and analyses (ANOVA with Tukey’s post hoc analysis) on fluorescent intensity of β-catenin expression normalized to total number of DAPI+ bEnd.3 cells per field in vehicle (white), RA (black), RARi (gray), RA+RARi (black and gray) treated cells ( n = 3 independent experiments).
    Figure Legend Snippet: Retinoic acid regulates β-catenin expression in brain endothelial cells. (A) Immunofluorescent images of coronal forebrain sections from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ) mutants stained for β-catenin (green), Isolectin-b4 (Ib4; red), and DAPI (blue). Scale bars are 200 μm. (B,C) Immunohistochemical images of (B) thalamic (thal) regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ; inset from A ) and Rdh10 ( Rdh10 -/- ; inset from A ) mutants and (C) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl brains stained for β-catenin (green) and Ib4 (red). Arrows indicate positive β-catenin expression within the Ib4 + vasculature. Scale bars are 50 μm. (B’,C’) Quantification and analyses (Student’s t -test) for percent of β-catenin expression within the total Ib4 + labeled blood vessels from (B’) Wild-type (gray; n = 6 animals) and Rdh10 mutant (black; n = 5 animals) and (C’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 5 animals). (D) Immunocytochemistry of β-catenin (green) expression in bEnd.3 cells following 48 h of vehicle, 50 nM RA, 1 μM RARi (AGN-194310), and RA + RARi. Scale bars are 50 μm. (D’) Quantification and analyses (ANOVA with Tukey’s post hoc analysis) on fluorescent intensity of β-catenin expression normalized to total number of DAPI+ bEnd.3 cells per field in vehicle (white), RA (black), RARi (gray), RA+RARi (black and gray) treated cells ( n = 3 independent experiments).

    Techniques Used: Expressing, Staining, Immunohistochemical staining, Labeling, Mutagenesis, Immunocytochemistry

    Ectopic vascular WNT signaling in RA mutants result in increased pericytes along the developing brain vasculature. (A–C) Immunohistochemical (IHC) images of the (A) thalamic regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ), (B) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl , and (C) cortical plate from E14.5 Ctnnb1 GOF/+ and Pdgfbi cre/+ ; Ctnnb1 GOF/+ brains stained for Pdgfrβ (green) and Isolectin-b4 (Ib4; blue). Enlarged images and arrows indicate Pdgfrβ + pericytes surrounding the Ib4 + vasculature. (A–C’) Quantification and analyses (Student’s t -test) for the number of pericytes co-labeled for Pdgfrβ and CoupTFII (staining not shown)/100 μm of Ib4 + blood vessels from (A’) W ild-type (gray; n = 5 animals) and Rdh10 mutants (black; n = 5 animals), (B’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 6 animals), and (C’) Ctnnb1 GOF/+ (gray; n = 4 animals) and Pdgfbi cre/+ ; Ctnnb1 GOF/+ (black; n = 4 animals). Scale bars are 50 μm.
    Figure Legend Snippet: Ectopic vascular WNT signaling in RA mutants result in increased pericytes along the developing brain vasculature. (A–C) Immunohistochemical (IHC) images of the (A) thalamic regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ), (B) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl , and (C) cortical plate from E14.5 Ctnnb1 GOF/+ and Pdgfbi cre/+ ; Ctnnb1 GOF/+ brains stained for Pdgfrβ (green) and Isolectin-b4 (Ib4; blue). Enlarged images and arrows indicate Pdgfrβ + pericytes surrounding the Ib4 + vasculature. (A–C’) Quantification and analyses (Student’s t -test) for the number of pericytes co-labeled for Pdgfrβ and CoupTFII (staining not shown)/100 μm of Ib4 + blood vessels from (A’) W ild-type (gray; n = 5 animals) and Rdh10 mutants (black; n = 5 animals), (B’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 6 animals), and (C’) Ctnnb1 GOF/+ (gray; n = 4 animals) and Pdgfbi cre/+ ; Ctnnb1 GOF/+ (black; n = 4 animals). Scale bars are 50 μm.

    Techniques Used: Immunohistochemical staining, Staining, Labeling

    Related Articles

    Mutagenesis:

    Article Title: Retinoic Acid Regulates Endothelial β-catenin Expression and Pericyte Numbers in the Developing Brain Vasculature
    Article Snippet: The following mouse lines were used in this study: PdgfbiCre , dnRAR403-flox , Ctnnb1-flox ( Ctnnb1 LOF ; ), Ctnnb1-exon3-flox ( Ctnnb1 GOF ; ), Cdh5Cre ERT2 (obtained from Ralf Adams), Sox17-flox , and Ai14-flox (Jackson Laboratories, Bar Harbor, ME, United States). .. The Rdh10 ENU point mutation mice were obtained from Andy Peterson at Genentech ( ). .. To activate Cre-mediated recombinase activity, Tamoxifen (Sigma, St. Louis, MO, United States) was dissolved in corn oil (Sigma, St. Louis, MO, United States; 20 mg/ml) and 100 mμl was injected intra-peritoneal into pregnant females at E9.5 and E10.5 to generate PdgfbiCre ; Ctnnb1 GOF , PdgfbiCre ; dnRAR403-flox , and Cdh5-CreER T2 ; Sox17-flox mutant animals.



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    rdh10 enu point mutation mice  (Genentech inc)
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    Genentech inc rdh10 enu point mutation mice
    Retinoic acid regulates β-catenin expression in brain endothelial cells. (A) Immunofluorescent images of coronal forebrain sections from E13.5 Wild-type ( <t>Rdh10</t> +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ) mutants stained for β-catenin (green), Isolectin-b4 (Ib4; red), and DAPI (blue). Scale bars are 200 μm. (B,C) Immunohistochemical images of (B) thalamic (thal) regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ; inset from A ) and Rdh10 ( Rdh10 -/- ; inset from A ) mutants and (C) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl brains stained for β-catenin (green) and Ib4 (red). Arrows indicate positive β-catenin expression within the Ib4 + vasculature. Scale bars are 50 μm. (B’,C’) Quantification and analyses (Student’s t -test) for percent of β-catenin expression within the total Ib4 + labeled blood vessels from (B’) Wild-type (gray; n = 6 animals) and Rdh10 mutant (black; n = 5 animals) and (C’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 5 animals). (D) Immunocytochemistry of β-catenin (green) expression in bEnd.3 cells following 48 h of vehicle, 50 nM RA, 1 μM RARi (AGN-194310), and RA + RARi. Scale bars are 50 μm. (D’) Quantification and analyses (ANOVA with Tukey’s post hoc analysis) on fluorescent intensity of β-catenin expression normalized to total number of DAPI+ bEnd.3 cells per field in vehicle (white), RA (black), RARi (gray), RA+RARi (black and gray) treated cells ( n = 3 independent experiments).
    Rdh10 Enu Point Mutation Mice, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rdh10+enu+point+mutation+mice/rdh10+enu+point+mutation+mutant+allele/pmc06290079-23-1-12
    Average 90 stars, based on 1 article reviews
    rdh10 enu point mutation mice - by Bioz Stars, 2026-09
    90/100 stars
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    Retinoic acid regulates β-catenin expression in brain endothelial cells. (A) Immunofluorescent images of coronal forebrain sections from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ) mutants stained for β-catenin (green), Isolectin-b4 (Ib4; red), and DAPI (blue). Scale bars are 200 μm. (B,C) Immunohistochemical images of (B) thalamic (thal) regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ; inset from A ) and Rdh10 ( Rdh10 -/- ; inset from A ) mutants and (C) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl brains stained for β-catenin (green) and Ib4 (red). Arrows indicate positive β-catenin expression within the Ib4 + vasculature. Scale bars are 50 μm. (B’,C’) Quantification and analyses (Student’s t -test) for percent of β-catenin expression within the total Ib4 + labeled blood vessels from (B’) Wild-type (gray; n = 6 animals) and Rdh10 mutant (black; n = 5 animals) and (C’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 5 animals). (D) Immunocytochemistry of β-catenin (green) expression in bEnd.3 cells following 48 h of vehicle, 50 nM RA, 1 μM RARi (AGN-194310), and RA + RARi. Scale bars are 50 μm. (D’) Quantification and analyses (ANOVA with Tukey’s post hoc analysis) on fluorescent intensity of β-catenin expression normalized to total number of DAPI+ bEnd.3 cells per field in vehicle (white), RA (black), RARi (gray), RA+RARi (black and gray) treated cells ( n = 3 independent experiments).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Retinoic Acid Regulates Endothelial β-catenin Expression and Pericyte Numbers in the Developing Brain Vasculature

    doi: 10.3389/fncel.2018.00476

    Figure Lengend Snippet: Retinoic acid regulates β-catenin expression in brain endothelial cells. (A) Immunofluorescent images of coronal forebrain sections from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ) mutants stained for β-catenin (green), Isolectin-b4 (Ib4; red), and DAPI (blue). Scale bars are 200 μm. (B,C) Immunohistochemical images of (B) thalamic (thal) regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ; inset from A ) and Rdh10 ( Rdh10 -/- ; inset from A ) mutants and (C) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl brains stained for β-catenin (green) and Ib4 (red). Arrows indicate positive β-catenin expression within the Ib4 + vasculature. Scale bars are 50 μm. (B’,C’) Quantification and analyses (Student’s t -test) for percent of β-catenin expression within the total Ib4 + labeled blood vessels from (B’) Wild-type (gray; n = 6 animals) and Rdh10 mutant (black; n = 5 animals) and (C’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 5 animals). (D) Immunocytochemistry of β-catenin (green) expression in bEnd.3 cells following 48 h of vehicle, 50 nM RA, 1 μM RARi (AGN-194310), and RA + RARi. Scale bars are 50 μm. (D’) Quantification and analyses (ANOVA with Tukey’s post hoc analysis) on fluorescent intensity of β-catenin expression normalized to total number of DAPI+ bEnd.3 cells per field in vehicle (white), RA (black), RARi (gray), RA+RARi (black and gray) treated cells ( n = 3 independent experiments).

    Article Snippet: The Rdh10 ENU point mutation mice were obtained from Andy Peterson at Genentech ( ).

    Techniques: Expressing, Staining, Immunohistochemical staining, Labeling, Mutagenesis, Immunocytochemistry

    Ectopic vascular WNT signaling in RA mutants result in increased pericytes along the developing brain vasculature. (A–C) Immunohistochemical (IHC) images of the (A) thalamic regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ), (B) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl , and (C) cortical plate from E14.5 Ctnnb1 GOF/+ and Pdgfbi cre/+ ; Ctnnb1 GOF/+ brains stained for Pdgfrβ (green) and Isolectin-b4 (Ib4; blue). Enlarged images and arrows indicate Pdgfrβ + pericytes surrounding the Ib4 + vasculature. (A–C’) Quantification and analyses (Student’s t -test) for the number of pericytes co-labeled for Pdgfrβ and CoupTFII (staining not shown)/100 μm of Ib4 + blood vessels from (A’) W ild-type (gray; n = 5 animals) and Rdh10 mutants (black; n = 5 animals), (B’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 6 animals), and (C’) Ctnnb1 GOF/+ (gray; n = 4 animals) and Pdgfbi cre/+ ; Ctnnb1 GOF/+ (black; n = 4 animals). Scale bars are 50 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Retinoic Acid Regulates Endothelial β-catenin Expression and Pericyte Numbers in the Developing Brain Vasculature

    doi: 10.3389/fncel.2018.00476

    Figure Lengend Snippet: Ectopic vascular WNT signaling in RA mutants result in increased pericytes along the developing brain vasculature. (A–C) Immunohistochemical (IHC) images of the (A) thalamic regions from E13.5 Wild-type ( Rdh10 +/+ or Rdh10 +/- ) and Rdh10 ( Rdh10 -/- ), (B) cortical plate from E18.5 dnRAR403 fl/fl and Pdgfbi cre/+ ; dnRAR403 fl/fl , and (C) cortical plate from E14.5 Ctnnb1 GOF/+ and Pdgfbi cre/+ ; Ctnnb1 GOF/+ brains stained for Pdgfrβ (green) and Isolectin-b4 (Ib4; blue). Enlarged images and arrows indicate Pdgfrβ + pericytes surrounding the Ib4 + vasculature. (A–C’) Quantification and analyses (Student’s t -test) for the number of pericytes co-labeled for Pdgfrβ and CoupTFII (staining not shown)/100 μm of Ib4 + blood vessels from (A’) W ild-type (gray; n = 5 animals) and Rdh10 mutants (black; n = 5 animals), (B’) dnRAR403 fl/fl (gray; n = 6 animals) and Pdgfbi cre/+ ; dnRAR403 fl/fl (black; n = 6 animals), and (C’) Ctnnb1 GOF/+ (gray; n = 4 animals) and Pdgfbi cre/+ ; Ctnnb1 GOF/+ (black; n = 4 animals). Scale bars are 50 μm.

    Article Snippet: The Rdh10 ENU point mutation mice were obtained from Andy Peterson at Genentech ( ).

    Techniques: Immunohistochemical staining, Staining, Labeling